王金 教授

教育和职业经历:

09/2000-07/2004        南京农业大学 生物科学专业                                理学学士学位

09/2004-07/2009        中科院上海生命科学研究院植物生理生态研究所   微生物学博士学位

07/2009-07/2012        香港中文大学微生物学系                                     博士后

07/2012-11/2013        中科院上海生命科学研究院植物生理生态研究所   助理研究员

12/2013-04/2018        中科院上海生命科学研究院植物生理生态研究所    副研究员

05/2018-05/2020         上海师范大学                                                     副研究员

06/2020-07/2022         深圳大学第一附属医院                                        教授

08/2022-                     上海吐露港生物科技有限公司                              CEO/CSO

研究方向：

主要研究方向是基于CRISPR原理的“下一代分子诊断技术的开发及其应用”。其中主要的成果包括：

（1）首次鉴定CRISPR-Cas12蛋白的反式切割活性，并开发了名为“HOLMES/福尔摩斯”的新型CRISPR分子诊断系统。

（2）开发了基于Cas12a的新型DNA体外无缝拼接/编辑技术，提高了大片段、复杂片段DNA的体外拼接/编辑效率和准确率。

（3）开发了基于Cas12a的新型生物元器件拼接标准（C-Brick）。

（4）开发了基于CRISPR技术的微生物基因编辑/基因调控系统，建成了不依赖于PAM位点的基因组编辑方法和基于CRISPR阵列的多基因高效转录调控的新方法。

近年来，申请人以第一作者或通讯作者身份在Cell Research、Nucleic Acids Research、ACS Synthetic Biology、Cell Discovery等杂志发表论文40多篇，并申请了多项发明专利。

近年主持的科研项目:

（1）国家自然科学基金优秀青年科学基金项目、“合成生物学新技术、新方法与新应用”（31922046）、2020年-2022年；

（2）国家自然科学基金面上项目、“分枝杆菌氮代谢信号转导途径中关键基因的筛选及其功能鉴定”（31770057）、2018年-2021年；

（3）中国科学院人才项目、“中国科学院青年创新促进会”（173176001000163003）、2017年-2018年。

发表论文 (# first author; * corresponding author):

2024年

1.Songkuan Zhuang^#, Tianshuai Hu^#, Hongzhong Zhou, Jie Li, Yu Zhao, Dayong Gu, Yong Xu*, Yijian Chen* and Jin Wang* (2024) CRISPR-HOLMES-based NAD⁺ detection. Frontiers in Bioengineering and Biotechnology, 12: 1355640; doi: 10.3389/fbioe.2024.1355640

2.Xinqiang Liu, Xinyun Wang, ZhiHui Shao, Jun Dang, Wei Wang, Chaoyue Liu, Jin Wang*, Hua Yuan*, and Guo-Ping Zhao (2024) The global nitrogen regulator GlnR is a direct transcriptional repressor of the key gluconeogenic gene pckA in actinomycetes. Journal of Bacteriology DOI: https://doi.org/10.1128/jb.00003-24

3.Songkuan Zhuang, Tianshuai Hu, Xike Zhou, Hongzhong Zhou, Shiping He, Jie Li, Long Qiu, Yuehui Zhang, Yong Xu, Hao Pei*, Dayong Gu* and Jin Wang* (2024) meHOLMES: A CRISPR-cas12a-based method for rapid detection of DNA methylation in a sequence-independent manner. Heliyon, 10: e24574. doi: https://doi.org/10.1016/ j.heliyon.2024.e24574.

2023年

4.Bo Gao, Guoqiang Li, Dayong Gu* and Jin Wang* (2023) Research Progress on GlnR-mediated Regulation in Actinomycetes. Front. Microbiol. 14:1282523. DOI: 10.3389/fmicb.2023.1282523

5.Linxian Li, Shiyuan Li, Dayong Gu, Yong Xu and Jin Wang* (2023) CRISPR-Cas12-Based Diagnostic Applications in Infectious and Zoonotic Diseases. Methods in Molecular Biology book series (MIMB,volume 2621, pp 267–278) DOI: 10.1007/978-1-0716-2950-5_15

6.Juanmei He^#, Xiaoman Kang^#, Jiacheng Wu, Zhihui Shao, Zhenhua Zhang, Yuqian Wu, Hua Yuan*, Guoping Zhao* and Jin Wang* (2023) Transcriptional Self-Regulation of the Master Nitrogen Regulator GlnR in Mycobacteria. J Bacteriol. e0047922. DOI: 10.1128/jb.00479-22.

2022年

7.Xiaoman Kang^#, Lei Yin^#, Songkuan Zhuang^#, Tianshuai Hu, Zhile Wu, Guoping Zhao, Yijian Chen*, Yong Xu* and Jin Wang* (2022) Reversible regulation of Cas12a activities by AcrVA5-mediated acetylation and CobB-mediated deacetylation. Cell Discovery 8: 45.

8.Guanglei Li^#, Xiangyang Li^#, Songkuan Zhuang^#, Liren Wang^#, Yifan Zhu^#, Yangcan Chen^#, Wen Sun^#, Zeguang Wu^#, Zhuo Zhou^#, Jia Chen*, Xingxu Huang*, Jin Wang*, Dali Li*, Wei Li*, Haoyi Wang*, Wensheng Wei* (2022) Gene Editing and Its Applications in Biomedicine. SCIENCE CHINA Life Sciences 65, doi: 10.1007/s11427-021-2057-0

2021年

9.Hailong Lv, Jian Wang, Jian Zhang, Yijian Chen, Lei Yin, Dian Jin, Dayong Gu*, Huailong Zhao*, Yong Xu* and Jin Wang* (2021) Definition of CRISPR Cas12 trans-cleavage units to facilitate CRISPR diagnostics. Front. Microbiol. 12:766464. doi: 10.3389/fmicb.2021.766464

10.Jian Zhang^#, Hailong Lv^#, Linxian Li, Minjie Chen, Dayong Gu, Jin Wang* and Yong Xu* (2021) Recent Improvements in CRISPR- Based Amplification-Free Pathogen Detection. Front. Microbiol. 12:751408. doi: 10.3389/fmicb.2021.751408

11.I Kuan Sam^#, Ying-Ying Chen^#, Jun Ma^#, Shi-Yuan Li^#, Ruo-Yan Ying, Lin-Xian Li, Ping Ji, Shu-Jun Wang, Jie Xu, Yu-Jie Bao, Guo-Ping Zhao, Hua-Jun Zheng, Jin Wang*, Wei Sha*, Ying Wang* (2021) TB-QUICK: CRISPR-Cas12b-assisted rapid and sensitive detection of Mycobacterium tuberculosis. J Infect. 2021 May 2; S0163-4453(21)00219-X.  doi: 10.1016/j.jinf.2021.04.032.

12.Ross Ka-Kit Leung^#, Qiuxiang Cheng^#, Zhile Wu^#, Gulfaraz Khan, Yang Liu, Hai-Yang Xia, Jin Wang* (2021) CRISPR-Cas12-based nucleic acids detection systems. Methods S1046-2023(21)00063-3.  doi: 10.1016/j.ymeth.2021.02.018.

-2020年

13.Weiren Huang^#, Lei Yu^#, Donghua Wen^#, Dong Wei^#, Yangyang Sun^#, Huailong Zhao, Yu Ye, Wei Chen, Yongqiang Zhu, Lijun Wang, Li Wang, Wenjuan Wu, Qianqian Zhao, Yong Xu, Dayong Gu, Guohui Nie, Dongyi Zhu, Zhongliang Guo, Xiaoling Ma, Liman Niu, Yikun Huang, Yuchen Liu, Bo Peng, Renli Zhang, Xiuming Zhang, Dechang Li, Yang Liu, Guoliang Yang, Lanzheng Liu, Yunying Zhou, Yunshan Wang, Tieying Hou, Qiuping Gao, Wujiao Li, Shuo Chen, Xuejiao Hu, Mei Han, Huajun Zheng, Jianping Weng, Zhiming Cai, Xinxin Zhang, Fei Song*, Guoping Zhao*, Jin Wang* (2020) A CRISPR-Cas12a-based specific enhancer for more sensitive detection of SARS-CoV-2 infection. EBioMedicine 61: 103036; DOI: doi.org/10.1016/j.ebiom.2020.103036

14.Yajun Zhou^#, Xinqiang Liu^#, Jiacheng Wu, Guoping Zhao, Jin Wang* (2020) CRISPR-Cas12a-assisted genome editing in Amycolatopsis mediterranei. Frontiers in Bioengineering and Biotechnology 8: 698; DOI: 10.3389/fbioe.2020.00698

15.Xinqiang Liu^#, Yuanyuan Liu^#, Chao Lei, Guoping Zhao, Jin Wang* (2020) GlnR dominates rifamycin biosynthesis by activating the rif cluster genes transcription both directly and indirectly in Amycolatopsis mediterranei. Frontiers in Microbiology 11: 319; doi.org/10.3389/fmicb.2020.00319

16.Linxian Li^#, Shiyuan Li^#, Na Wu^#, Jiacheng Wu, Gang Wang, Guoping Zhao, Jin Wang* (2019) HOLMESv2: a CRISPR-Cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation. ACS Synthetic Biology 8(10):2228-2237.

17.Ran Zhao, Yanqiang Liu, Huan Zhang, Changsheng Chai, Jin Wang*, Weihong Jiang*, Yang Gu* (2019) CRISPR-Cas12a-mediated gene deletion and regulation in Clostridium ljungdahlii and its application in carbon flux redirection in synthesis gas fermentation. ACS Synthetic Biology 8(10): 2270-2279.

18.Anrui Lu^#, Jingman Wang^#, Weihong Sun*, Weiren Huang*, Zhiming Cai*, Guoping Zhao, Jin Wang* (2019) Reprogrammable CRISPR/dCas9-based recruitment of DNMT1 for site-specific DNA demethylation and gene regulation. Cell Discovery 5: 22.

19.Jingman Wang^#, Anrui Lu^#, Jinxin Bei*, Weiren Huang, Zhiming Cai, Guoping Zhao*, Jin Wang* (2019) CRISPR/ddCas12a-based programmable and accurate gene regulation. Cell Discovery 5: 15.

20.Yi Li^#, Shi-Yuan Li^#, Jin Wang*, Guo-Zhen Liu* (2018) CRISPR/Cas Systems towards Next-Generation Biosensing. Trends in Biotechnology 37(7): 730-743; DOI:https://doi.org/10.1016/j.tibtech.2018.12.005

21.Shi-Yuan Li, Jia-Kun Liu, Guo-Ping Zhao and Jin Wang* (2018) CADS: CRISPR/Cas12a-Assisted DNA Steganography for Securing the Storage and Transfer of DNA-encoded Information. ACS Synthetic Biology 7(4):1174-1178.

22.Shi-Yuan Li^#, Qiu-Xiang Cheng^#, Jing-Man Wang, Xiao-Yan Li, Zi-Long Zhang, Song Gao, Rui-Bing Cao, Guo-Ping Zhao and Jin Wang* (2018) CRISPR-Cas12a-assisted nucleic acid detection. Cell Discovery 4: 20.

23.Zhendong Li^#, Xinqiang Liu^#, Jingzhi Wang, Ying Wang, Guosong Zheng, Yinhua Lu, Guoping Zhao, Jin Wang* (2018) Insight into the molecular mechanism of the transcriptional regulation of amtB operon in Streptomyces coelicolor. Frontiers in Microbiology 9: 264

24.Shi-Yuan Li, Qiu-Xiang Cheng*, Jia-Kun Liu, Xiao-Qun Nie, Guo-Ping Zhao and Jin Wang* (2018) CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA. Cell Research 28: 491–493.

25.Chao Lei^#, Jingzhi Wang^#, Yuanyuan Liu, Xinqiang Liu, Guoping Zhao, Jin Wang* (2018) A feedback regulatory model for RifQ-mediated repression of rifamycin export in Amycolatopsis mediterranei. Microbial Cell Factories 17:14

26.Xiaochun Zhang^#, Jingman Wang^#, Qiuxiang Cheng^#, Xuan Zheng, Guoping Zhao, Jin Wang* (2017) Multiplex gene regulation by CRISPR–ddCpf1. Cell Discovery 3: 17018

27.Hong Zhang, Qiuxiang Cheng, Aimin Liu*, Guoping Zhao, Jin Wang* (2017) A novel and efficient method for bacteria genome editing employing both CRISPR/Cas9 and an antibiotic resistance cassette. Frontiers in Microbiology 8: 812

28.ShiYuan Li, GuoPing Zhao, Jin Wang* (2017) Protocols for C-Brick DNA standard assembly using Cpf1. Journal of Visualized Experiments (124), e55775

29.Xuan Zheng^#, ShiYuan Li^#, GuoPing Zhao, Jin Wang* (2017) An efficient system for deletion of large DNA fragments in Escherichia coli via introduction of both Cas9 and the non-homologous end joining system from Mycobacterium smegmatis. Biochemical and Biophysical Research Communications 485(4): 768-774

30.Chen Li^#, XinQiang Liu^#, Chao Lei, Han Yan, ZhiHui Shao, Ying Wang, GuoPing Zhao, Jin Wang*, XiaoMing Ding* (2017) RifZ (AMED_0655) is a pathway-specific regulator for rifamycin biosynthesis in Amycolatopsis mediterranei. Applied Environmental Microbiology 83(8): e03201-16

31.Chao Lei^#, ShiYuan Li^#, JiaKun Liu^#, Xuan Zheng, GuoPing Zhao, Jin Wang* (2017) The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro. Nucleic Acids Research 45(9): e74.

32.ShiYuan Li, GuoPing Zhao*, Jin Wang* (2016) C-Brick: A New Standard for Assembly of Biological Parts Using Cpf1. ACS Synthetic Biology 5(12):1383-1388.

33.XuFeng Cen, JingZhi Wang, GuoPing Zhao, Ying Wang*, Jin Wang* (2016) Molecular evidence for the coordination of nitrogen and carbon metabolisms, revealed by a study on the transcriptional regulation of the agl3EFG operon that encodes a putative carbohydrate transporter in Streptomyces coelicolor. Biochemical and Biophysical Research Communications 471(4):510-514.

34.ZhiHui Shao^#, WanXin Deng^#, ShiYuan Li, JuanMei He, ShuangXi Ren, WeiRen Huang, YinHua Lu, GuoPing Zhao, ZhiMing Cai*, Jin Wang* (2015) GlnR-Mediated Regulation of ectABCD Transcription Expands the Role of the GlnR Regulon to Osmotic Stress Management. Journal of Bacteriology 197(19): 3041–3047.

35.ZhuiHui Shao, ShuangXi Ren, XinQiang Liu, Jian Xu, Han Yan, GuoPing Zhao, Jin Wang* (2015) A preliminary study of the mechanism of nitrate-stimulated remarkable increase of rifamycin production in Amycolatopsis mediterranei U32 by RNA-seq. Microbial Cell Factories 14: 75.

36.Jin Wang*, Ying Wang, GuoPing Zhao (2015) Precise characterization of GlnR Box in actinomycetes. Biochemical and Biophysical Research Communications 458(3): 605-607.

37.JiaKun Liu, WeiHua Chen, ShuangXi Ren, GuoPing Zhao*, Jin Wang*. (2014) iBrick: a new standard for iterative assembly of biological parts with homing endonucleases. PLoS One 9(10): e110852.

38.Ying Wang^#, Chen Li^#, Na Duan^#, Bing Li, XiaoMing Ding, YuFeng Yao, Jun Hu*, GuoPing Zhao*, Jin Wang*. (2014) GlnR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterranei U32 under Nitrogen Limited Conditions via Specific Binding to Its Major Transcription Initiation Site. PLoS One 9(8): e104811.

39.Ying Wang, JingZhi Wang, ZhiHui Shao, Hua Yuan, YinHua Lu, WeiHong Jiang, GuoPing Zhao*, Jin Wang* (2013) Three of Four GlnR Binding Sites Are Essential for GlnR-Mediated Activation of Transcription of the Amycolatopsis mediterranei nas Operon. Journal of Bacteriology 195(11): 2595-2602.

40.WeiHua Chen, ZhongJun Qin, Jin Wang* and GuoPing Zhao* (2013) The MASTER (Methylation-Assisted Tailorable Ends Rational) Ligation method for seamless DNA assembly. Nucleic Acids Research 41(8): e93.

41.Ying Wang, XuFeng Cen, GuoPing Zhao* and Jin Wang* (2012) Characterization of a New GlnR Binding Box in the Promoter of amtB in Streptomyces coelicolor Inferred a PhoP/GlnR Competitive Binding Mechanism for Transcriptional Regulation of amtB. Journal of Bacteriology 194(19): 5237-5244.

42.Wei Zhao^#, Yi Zhong^#, Hua Yuan^#, Jin Wang^#, et al. (2010) Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism. Cell Research 20(10):1096-1108.

43.Jin Wang and Guoping Zhao*. (2009) GlnR positively regulates nasA transcription in Streptomyces coelicolor. Biochemical and Biophysical Research Communications 386 (1): 77-81.

44.李诗渊^#，赵国屏，王金*. 合成生物学技术的研究进展——DNA合成、组装与基因组编辑。《生物工程学报》2017, 33(3): 1−17

45.李诗渊^#，赵国屏，王金*. CRISPR技术在生物传感中的应用——胞内核酸成像、体外核酸检测与胞内DNA记录。《生物产业技术》2019, 1: 75-83

46.刘晓^#，王慧媛，熊燕，赵国屏，王金*. 基因合成与基因组编辑。《中国细胞生物学学报》2019，41(11): 2072-2082

47.赵东东、宗媛、尹蕾、毕昌昊*、王金*、高彩霞*、张学礼*. 基因组编辑技术及未来发展。《生命科学》2021, 33(12): 1462-1468

联系方式：

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